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rabbit polyclonal anti spike s2 antibody  (Sino Biological)


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    Sino Biological rabbit polyclonal anti spike s2 antibody
    Rabbit Polyclonal Anti Spike S2 Antibody, supplied by Sino Biological, used in various techniques. Bioz Stars score: 96/100, based on 152 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+spike+polyclonal+antibody/SARS-CoV-2+(2019-nCoV)+Spike+S2+Antibody%2C+Rabbit+PAb%2C+Antigen+Affinity+Purified/pm41549568-251-38-43
    Average 96 stars, based on 152 article reviews
    rabbit polyclonal anti spike s2 antibody - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Immunoprecipitation:

    Article Title: Preclinical development of a cross-protective β-SARS-CoV-2 virus-like particle vaccine adjuvanted with MF59
    Article Snippet: .. VLPs were immunoprecipitated with a murine RBD-specific monoclonal antibody and detected with a rabbit anti-spike polyclonal antibody (40591-T62, Sino Biologicals, China) essentially as previously described . .. Briefly, ß-S 13 EM-SARS-CoV-2 VLPs were incubated at 4 °C overnight with murine anti-RBD monoclonal antibody (clone 20-14-5) coupled to protein A sepharose, (or murine anti-SARS-CoV-2 nucleoprotein monoclonal antibody as negative control), centrifuged at 17,000 g for 5 min at 4 °C and the pellets washed twice (0.5 ml 0.05% Triton X-100, 10 mM Tris-HCl (pH 7.5), 2 mM EDTA in 150 mM NaCl which was subsequently decreased to 0.5 mM NaCl) and a final wash in 10 mM Tris-HCl, pH 7.5.

    Article Title: Preclinical development of a cross-protective β-SARS-CoV-2 virus-like particle vaccine adjuvanted with MF59.
    Article Snippet: .. VLPs were immunoprecipitated with a murine RBD-specific monoclonal antibody and detected with a rabbit anti-spike polyclonal antibody (40591-T62, Sino Biologicals, China) essentially as previously described 87. .. Briefly, ß-S13EM-SARS-CoV-2 VLPs were incubated at 4 °C overnight with murine anti-RBD monoclonal antibody (clone 20-14-5) coupled to protein A sepharose, (or murine anti-SARS-CoV-2 nucleoprotein monoclonal antibody as negative control), centrifuged at 17,000 g for 5 minutes at 4 °C and the pellets washed twice (0.5 ml 0.05 % Triton X-100, 10 mM Tris-HCl (pH 7.5), 2mM EDTA in 150 mM NaCl which was subsequently decreased to 0.5 mM NaCl) and a final wash in 10 mM Tris-HCl, pH 7.5.

    Article Title: Preclinical evaluation of a cross-protective ß-SARS-CoV-2 virus-like particle vaccine adjuvanted with MF59
    Article Snippet: .. VLPs were immunoprecipitated with a murine RBD-specific monoclonal antibody and detected with a rabbit anti-spike polyclonal antibody (40591-T62, Sino Biologicals, China) 630 essentially as previously described 71(Supplementary methods). .. C57BL/6 mice were bred in-house at the Peter Doherty Institute for Infection and Immunity, Biological Research Facility, Melbourne, Australia.

    Article Title: Preclinical evaluation of a cross-protective ß-SARS-CoV-2 virus-like particle vaccine adjuvanted with MF59
    Article Snippet: File type conversion and morphometry were performed using the FEI TIA 965 software package (56). .. VLPs were immunoprecipitated with a murine RBD-specific monoclonal antibody and detected with a rabbit anti-spike polyclonal antibody (40591-T62, Sino Biologicals, China) essentially as previously described 71. .. Briefly, ß-S13EM-SARS-CoV-2 VLPs were incubated at 970 4 °C overnight with murine anti-RBD monoclonal antibody (clone 20-14-5) coupled to protein A sepharose, (or murine anti-SARS-CoV-2 nucleoprotein monoclonal antibody as negative control), centrifuged at 17,000 g for 5 minutes at 4 °C and the pellets washed twice (0.5 ml 0.05 % Triton X-100, 10 mM Tris-HCl (pH 7.5), 2mM EDTA in 150 mM NaCl which was subsequently decreased to 0.5 mM NaCl) and a final wash in 10 mM Tris-HCl, pH 7.5.

    Incubation:

    Article Title: Severe acute respiratory syndrome coronavirus 2 virus-like particles induce dendritic cell maturation and modulate T cell immunity
    Article Snippet: .. The grids were incubated with rabbit anti-spike polyclonal antibody (1:50 dilution; SinoBiological, China) at room temperature for 1 h, followed by treatment with goat anti-rabbit immunoglobulin G (1:20 dilution) (whole)-gold conjugate (10 nm) (BOSTER, China). ..

    Article Title: Sequential Immunization with Vaccines Based on SARS-CoV-2 Virus-like Particles Induces Broadly Neutralizing Antibodies
    Article Snippet: .. Purified VLPs preparations were adsorbed onto 200 mesh carbon-coated copper grids for 3 min, which were then incubated with rabbit anti-spike polyclonal antibody (40591-T62, SinoBiological, Beijing, China) for 1 h at room temperature. .. After washing the grids three times, the colloidal gold conjugated goat anti-mouse IgG (10 nm) (BOSTER, Wuhan, China) was incubated on the grid for another hour.

    other:

    Article Title: Syntaxin-6 restricts SARS-CoV-2 infection by facilitating virus trafficking to autophagosomes.
    Article Snippet: Rabbit anti-spike polyclonal antibody (40589-T62) and mouse anti-nucleocapsid monoclonal antibody (40143-MM05) were obtained from Sino Biological.

    Article Title: Syntaxin-6 restricts SARS-CoV-2 infection by facilitating virus trafficking to autophagosomes
    Article Snippet: Rabbit anti-spike polyclonal antibody (40589-T62) and mouse anti-nucleocapsid monoclonal antibody (40143-MM05) were obtained from Sino Biological.

    Purification:

    Article Title: Sequential Immunization with Vaccines Based on SARS-CoV-2 Virus-like Particles Induces Broadly Neutralizing Antibodies
    Article Snippet: .. Purified VLPs preparations were adsorbed onto 200 mesh carbon-coated copper grids for 3 min, which were then incubated with rabbit anti-spike polyclonal antibody (40591-T62, SinoBiological, Beijing, China) for 1 h at room temperature. .. After washing the grids three times, the colloidal gold conjugated goat anti-mouse IgG (10 nm) (BOSTER, Wuhan, China) was incubated on the grid for another hour.



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    Reagents for analysing HCoV-OC43. A. Western blot of Mv.1.Lu cells infected with HCoV-OC43 at an MOI of 10 PFU/cell and harvested at the indicated time points, probed with two anti-Nucleocapsid (N) antibodies, derived from Sheep (Sh) polyclonal antiserum from the MRC PPU & CVR Coronavirus toolkit and a commercial Rabbit (Rb) polyclonal antiserum. Host HSP90 is included as a loading control. B . Immunofluorescence microscopy of HCoV-infected A549 cells, stained with Sheep anti-N and anti double-stranded RNA (dsRNA), a marker of RNA replication. C . Immunoprecipitation (IP) of lysates from HCoV-OC43-infected cells (MOI 3, 24 hpi) using Sheep anti-N and probed with Sheep anti-N <t>and</t> <t>anti-Spike</t> (S). Host HSP90 is included as a loading control. D . Immunofluorescence microscopy of HCoV-OC43-infected A549 cells, stained with mouse monoclonal (Mm) or rabbit polyclonal (Rb) anti-S. E . Silver stain (SS) and western blotting (WB) of virions purified by ultracentrifugation through a 30% sucrose cushion. The supernatant (sup) before and after sucrose cushion, and the pellet, containing purified virions, are shown. Viral structural proteins are indicated by red arrowheads and BSA from cell culture medium by a red asterisk. F . Immunofluorescence microscopy of HCoV-OC43-infected A549 cells stained with anti-SARS-CoV M. Scale bars represent 10 µm.
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    rabbit polyclonal anti sars cov 2 spike antibody  (Sino Biological)
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    Reagents for analysing HCoV-OC43. A. Western blot of Mv.1.Lu cells infected with HCoV-OC43 at an MOI of 10 PFU/cell and harvested at the indicated time points, probed with two anti-Nucleocapsid (N) antibodies, derived from Sheep (Sh) polyclonal antiserum from the MRC PPU & CVR Coronavirus toolkit and a commercial Rabbit (Rb) polyclonal antiserum. Host HSP90 is included as a loading control. B . Immunofluorescence microscopy of HCoV-infected A549 cells, stained with Sheep anti-N and anti double-stranded RNA (dsRNA), a marker of RNA replication. C . Immunoprecipitation (IP) of lysates from HCoV-OC43-infected cells (MOI 3, 24 hpi) using Sheep anti-N and probed with Sheep anti-N <t>and</t> <t>anti-Spike</t> (S). Host HSP90 is included as a loading control. D . Immunofluorescence microscopy of HCoV-OC43-infected A549 cells, stained with mouse monoclonal (Mm) or rabbit polyclonal (Rb) anti-S. E . Silver stain (SS) and western blotting (WB) of virions purified by ultracentrifugation through a 30% sucrose cushion. The supernatant (sup) before and after sucrose cushion, and the pellet, containing purified virions, are shown. Viral structural proteins are indicated by red arrowheads and BSA from cell culture medium by a red asterisk. F . Immunofluorescence microscopy of HCoV-OC43-infected A549 cells stained with anti-SARS-CoV M. Scale bars represent 10 µm.
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    https://www.bioz.com/product/rabbit+anti+spike+polyclonal+antibody/SARS-CoV-2+(2019-nCoV)+Spike+Antibody%2C+Rabbit+PAb%2C+Antigen+Affinity+Purified/pm41170287-86-3-10
    Average 93 stars, based on 1 article reviews
    rabbit polyclonal anti sars cov 2 spike antibody - by Bioz Stars, 2026-09
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    Image Search Results


    Reagents for analysing HCoV-OC43. A. Western blot of Mv.1.Lu cells infected with HCoV-OC43 at an MOI of 10 PFU/cell and harvested at the indicated time points, probed with two anti-Nucleocapsid (N) antibodies, derived from Sheep (Sh) polyclonal antiserum from the MRC PPU & CVR Coronavirus toolkit and a commercial Rabbit (Rb) polyclonal antiserum. Host HSP90 is included as a loading control. B . Immunofluorescence microscopy of HCoV-infected A549 cells, stained with Sheep anti-N and anti double-stranded RNA (dsRNA), a marker of RNA replication. C . Immunoprecipitation (IP) of lysates from HCoV-OC43-infected cells (MOI 3, 24 hpi) using Sheep anti-N and probed with Sheep anti-N and anti-Spike (S). Host HSP90 is included as a loading control. D . Immunofluorescence microscopy of HCoV-OC43-infected A549 cells, stained with mouse monoclonal (Mm) or rabbit polyclonal (Rb) anti-S. E . Silver stain (SS) and western blotting (WB) of virions purified by ultracentrifugation through a 30% sucrose cushion. The supernatant (sup) before and after sucrose cushion, and the pellet, containing purified virions, are shown. Viral structural proteins are indicated by red arrowheads and BSA from cell culture medium by a red asterisk. F . Immunofluorescence microscopy of HCoV-OC43-infected A549 cells stained with anti-SARS-CoV M. Scale bars represent 10 µm.

    Journal: bioRxiv

    Article Title: A stable subgenomic reporter coronavirus enables transcriptional profiling of bystander cells

    doi: 10.64898/2026.02.27.708290

    Figure Lengend Snippet: Reagents for analysing HCoV-OC43. A. Western blot of Mv.1.Lu cells infected with HCoV-OC43 at an MOI of 10 PFU/cell and harvested at the indicated time points, probed with two anti-Nucleocapsid (N) antibodies, derived from Sheep (Sh) polyclonal antiserum from the MRC PPU & CVR Coronavirus toolkit and a commercial Rabbit (Rb) polyclonal antiserum. Host HSP90 is included as a loading control. B . Immunofluorescence microscopy of HCoV-infected A549 cells, stained with Sheep anti-N and anti double-stranded RNA (dsRNA), a marker of RNA replication. C . Immunoprecipitation (IP) of lysates from HCoV-OC43-infected cells (MOI 3, 24 hpi) using Sheep anti-N and probed with Sheep anti-N and anti-Spike (S). Host HSP90 is included as a loading control. D . Immunofluorescence microscopy of HCoV-OC43-infected A549 cells, stained with mouse monoclonal (Mm) or rabbit polyclonal (Rb) anti-S. E . Silver stain (SS) and western blotting (WB) of virions purified by ultracentrifugation through a 30% sucrose cushion. The supernatant (sup) before and after sucrose cushion, and the pellet, containing purified virions, are shown. Viral structural proteins are indicated by red arrowheads and BSA from cell culture medium by a red asterisk. F . Immunofluorescence microscopy of HCoV-OC43-infected A549 cells stained with anti-SARS-CoV M. Scale bars represent 10 µm.

    Article Snippet: Membranes were probed with sheep polyclonal anti-Nucleocapsid anti-serum (MRC PPU & CVR Coronavirus Toolkit, Sheep No. DA116, 1:1000) , rabbit anti-Nucleocapsid (40643-T62, Sino Biological, 1:1000), anti-Spike (PAB21478-100, The Native Antigen Company, 1:1000), anti-SARS-CoV Membrane (ABIN1887462, Antibodies Online, 1:200), anti-MHV nucleocapsid J3.3 ( ) (1:200), anti-GAPDH (AM4300, Invitrogen, 1:4000) and anti-HSP90 (MA5-35624, Invitrogen, 1:2000).

    Techniques: Western Blot, Infection, Derivative Assay, Control, Immunofluorescence, Microscopy, Staining, Marker, Immunoprecipitation, Silver Staining, Purification, Cell Culture